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2017-03-02 来源:亚科官网
2 March 2017
  Trimethylolaminomethane (Tris), is an important biological buffer and standard of acid titration, has a very wide range of social use. There are more information in the field of biochemistry and medicine, but in fact Tris is also widely used in the many studies, this article will summarize the use of Tris.
Biological buffers
  The most well-known application of Tris is the use of solvents for nucleic acids and proteins and biological buffers. Tris-HCl, NaCl, tween20, These three substances can be composed of TBST buffer, which is the buffer commonly used in Western Blotting; by adding EDTA in Tris-HCl can make TE buffer, which is used for DNA stability and storage; change the solution of adjusting the pH value into acetic acid to get TAE buffer, use boric acid to obtained TBE buffer, the two buffers commonly used in nucleic acid electrophoresis experiments.
  Tris is alkaline, only Tris-HCl buffer system can prepare buffer from acidic to alkaline, not only the method is relatively simple, but also can form a variety of buffer system with other substances; it has very small effects on the biochemical process, and not react with calcium, magnesium ions and heavy metal ions. However, the pH value of Tris buffer is greatly affected by the solution concentration and temperature, diluted ten times, the pH value of the buffer changes more than 0.1, the temperature is increased once every time, the pH value decreases 0.03; It is easy to absorb CO2, so the buffer should be sealed after preparation.
  Surfactant refers to the addition that can make the interface of the solution system significantly change by a small amount with a fixed hydrophilic lipophilic group, it can be aligned on the surface of the solution. The surfactants can be divided into civil and industrial applications. 2/3 of the civilian surfactants are used for personal protection articles. Industrial surfactants are the sum of the surfactants used in the industrial field other than the civil surfactants.
  The molecular structure of the surfactant is amphiphilic: one end is a hydrophilic group and the other end is a hydrophobic group; the hydrophilic group is often a polar group such as carboxylic acid, sulfuric acid, amino and its salts, hydroxyl groups, amide groups, ether bonds and the like can also be used as polar hydrophilic groups; and hydrophobic groups are often non-polar hydrocarbon chains, such as hydrocarbon chains above 8 carbon atoms. Tris contains three hydroxyl groups and one amino group, all are good hydrophilic groups, so Tris is also an important intermediate for the synthesis of surfactants.
Vulcanization accelerator
  The vulcanization accelerator is a substance that promotes vulcanization, shortens the vulcanization time, reduces the vulcanization temperature, reduces the amount of vulcanizing agent and improves the physical and mechanical properties of the rubber. Tris-based accelerators are mainly used for hardening of resins and coatings; also used in printed circuit board inks, electrically insulating materials, resin molds, screen printing coatings, molds, adhesives, linings Materials and plastic stabilizers.
Drugs and intermediates
  In the field of medicine, Tris's application is mainly divided into pharmaceutical intermediates and medicine. Tris is the fosfomycin tromethamine intermediates, fosfomycin tromethamine is a broad-spectrum antimicrobial agents listed in the late eighties, its bioavailability is 3~6 times of commonly used antimicrobial fosfomycin calcium, and more suitable for the treatment of urinary tract infections and other diseases.
  Tris is also a drug for the treatment of blood diseases, can be used for acute metabolic acidosis and acute respiratory acidosis. As a sodium-free amino-buffered base, it binds to H2CO3 in body fluids to form bicarbonate. So it can buffer acid and reduce CO2 tension, and has the double effect to correct acute respiratory acidosis and metabolic acidosis. It is faster and stronger than sodium bicarbonate, especially for people with heart failure. It is also suitable for the rescue of low-temperature anesthesia, shock and cardiac arrest, and ischemic intracellular acidosis after organ transplantation.
Chemical reducing agent
  Tris has three hydroxyl in equal status, which can be used as a good reducing agent. Researchers use Tris as a reducing agent to prepare gold nanoparticles in the alkaline conditions through the reduction of chloroauric acid solution without adding other stabilizers. It achieves the purpose of synthesis for biocompatible gold nanoparticles environmental friendly. The experimental conditions are mild, easy to operate, and non pollution with low toxicity and low cost.
  Tris is widely used from the laboratory of biochemical experiments to the industrialization, and then to daily items. As for toxicity, it is a piece of cake compared to some frightening reagents, but it also need the prevention. Tris can harm the body by inhalation, ingestion, and skin absorption. Therefore, it is an effective protective measure for the laboratory personnel to wear gloves and goggles.
Related Links: Tris
                      Comparison of Tris Buffer Solution with Phosphate Buffer Solution
Edited by the Editorial Office of Suzhou Yacoo Science Co., Ltd.