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Application of D-trehalose|CAS:6138-23-4| in heparin detection kit

2022-09-19 来源:转载自第三方

D-trehalose is an organic compound with a molecular formula of C12H22O11·2H2O and a molecular weight of 378.3. Trehalose has stable biofilm and protein structure, and is mainly used in the protection of biological products, such as genetically engineered enzymes and other enzymes, cell membranes, antibodies, antigens, organelles, etc. In addition, trehalose has the effect of inhibiting the decomposition of collagen. Therefore, it can be used to improve osteoporosis. CN109709292A patent introduces a heparin detection kit and its application. The heparin detection kit includes four components: heparinase I, anhydrous calcium chloride, glycerol and D-trehalose. The preparation method is to mix heparinase I, anhydrous calcium chloride, glycerol and D-trehalose, and its specific composition is as follows:

Heparinase I: Heparinase I is a liquid enzyme preparation, and the concentration of heparinase I is 60%-80% (5IU/ml-10IU/ml);

Anhydrous calcium chloride: the concentration is 0.05%-0.4%;

Glycerol: the concentration is 0.2%-0.8%;

D-trehalose: the concentration is 0.2%-0.8%;

The heparin detection kit can quickly and specifically neutralize the anticoagulant activity of heparin without affecting other blood components of coagulation, so as to more accurately and quickly evaluate the residual situation of heparin sodium salt and evaluate the anticoagulant effect of heparin sodium salt, Provide a basis for clinical medication.


CN109709292A A heparin detection kit and its application.


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