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CAS:593-85-1|Application of guanidine carbonate in biochemistry
Product Name/English Name:Guanidine carbonate
CAS:593-85-1
Molecular Formula: C2H10N6H2CO3
Structural Formula:
Product Introduction
Guanidine carbonate is an extremely versatile organic synthetic raw material and analytical reagent, mainly used as a pH regulator, antioxidant, resin stabilizer, and soap for amino resins. It is also used as an additive for cement slurries and surfactants. It can also be used as a moisture resistant and synergistic agent for detergents, cosmetic raw materials, etc.
Application Field of Guanidine carbonate
Nucleic acid extraction and purification is a fundamental operation in molecular biology. At present, in molecular biology or clinical gene diagnosis, due to the high purity of nucleic acid extracted by centrifugal column method and magnetic bead method, as well as the ability to achieve automated operation, it has the advantages of efficient, convenient, and high-throughput extraction, which is the future development direction of nucleic acid extraction in clinical diagnosis. In practical operations, due to the effect of guanidine salts on reducing the pH value of the solution, the guanidine salts in these cell lysates are prone to precipitate under conditions with pH values near neutral or slightly acidic, which not only makes it inconvenient for reagents to be heated for dissolution and extraction, but also affects the cell lysis effect in the sample and reduces the yield of nucleic acid extraction. Therefore, CN102533724B patent provides a cell lysis reagent for the extraction and purification of nucleic acid in biological samples, which is mainly composed of guanidine salt, ammonium ion and surfactant, wherein guanidine salt can be guanidine isothiocyanate, Guanidinium chloride, guanidine carbonate, guanidine phosphate, etc.
The guanidine salt in this patent reagent is a strong denaturant for proteins and nucleic acids, and a promoter for the binding adsorption of nucleic acids and silica based solid phase materials. Especially due to the synergistic effect of the selected key component ammonium ions and surfactants, it can not only fully lyse cells, separate denatured nucleic acid molecules from denatured protein molecules that have lost their secondary structure, but also maintain the stability of nucleic acid in the lysis mixture, It can be widely used for the extraction and purification of nucleic acid from different types of biological samples such as serum, plasma, saliva, and urine.
References
CN102533724B A cell lysis reagent for nucleic acid extraction and purification in biological samples.