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Physical Properties and Applications of IPTG (367-93-1)

2015-08-13 来源:转载自第三方
Key words: isopropyl thiogalactoside; IPTG
The chemical name of IPTG (367-93-1) is isopropyl-β-D-thiogalactoside, also known as isopropylthiogalactoside, which is an inducer of β-galactosidase activity. IPTG is a white crystalline powder with a melting point of 105°C and a specific rotation of -28.5° to -34.5°. As a polar substance, IPTG is soluble in water and methanol and acts as an active inducer of β-galactosidase for bioactivity in applications Identification. The stability of IPTG is poor, so its crystal need to be stored at 2~8°C in the refrigeration, prepare and store at -20°C, can be placed at room temperature for a month. As an active inducer of β-galactosidase, IPTG is often used in cloning experiments. It is often used in combination with X-Gal or Bluo-Gal for blue-white screening of recombinant bacterial colonies which can induce the expression of the lac operon in E. coli. IPTG binds to the lacI repressor protein and alters its conformation, preventing the inhibition of the lacZ gene encoding β-galactosidase.
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Custom synthesis, diagnostic reagents, biochemical reagents, intermediate, BPC, electronic chemicals


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