Application of MOPS|CAS：1132-61-2| in in vitro diagnosis

MOPS is an organic compound with molecular formula of C₇H₁₅NO₄S and molecular weight of 209.26. MOPS is an important biological buffer, which is often used to prepare RNA electrophoresis buffer. It can not only be used as a buffer system of cell culture medium, but also a common electrophoresis buffer and protein purification chromatography buffer. CN107167356B patent introduces a dye that can quickly develop color after cell staining in urine and its use method. MOPS is used as a buffer in this dye, and its specific technical scheme is as follows:

A dye for rapid color development after cell staining in urine includes reagent A and reagent B, and the mass ratio of reagent A and reagent B is 0.25-4.

Reagent A includes 0.6～1.2g neutral red dye and 100g dye solvent;

Reagent B includes a buffer of 10mM～600mM, a cell membrane damage agent of 0.1～8g, and a pH regulator of 0.01～10g. The buffering agent is 2 - (N-morpholine) ethanesulfonic acid, 1,3-di [(trimethylolmethyl) methylamino] propane, piperazin-1,4-diethylsulfonic acid, MOPS, 3 - (N-morpholinyl) 2-hydroxypropane sulfonic acid, disodium hydrogen phosphate citric acid, disodium hydrogen phosphate sodium dihydrogen phosphate, disodium hydrogen phosphate potassium dihydrogen phosphate, potassium dihydrogen phosphate sodium hydroxide, trimethylolaminomethane hydrochloric acid, sodium barbital hydrochloric acid, sodium propionate sodium propionate One or more mixtures of acetic acid sodium acetate;

The fast coloration reagent provided by the patent can directly dye cells in urine without pretreatment, and has short coloration time, simple operation and high detection efficiency; In addition, the stain does not affect the morphology of red blood cells with pathological significance, and will not lead to aggregation of high concentration red blood cell samples, which is conducive to improving the accuracy of detection results and achieving the purpose of automatic detection.

Reference

CN107167356B A dye for rapid color development of cells in urine after dyeing and its application method.