CAS：68399-77-9| The Application of MOPSO in complement proteins

Product Name：MOPSO
CAS：    68399-77-9
Molecular Formula：C7H15NO5S
Article No.：    M0003
Structural Formula：

Product Introduction
Mopso is a zwitterionic bio buffering reagent with a structure similar to MOPS and an effective pH range of 6.2-7.6. MOPSO is mainly used as a biological buffer for biochemical research.
Application of MOPSO
Complement C4 is a multifunctional substance β 1-Globulin, present in plasma. The detection of complement C4 has diagnostic and therapeutic value for autoimmune diseases such as systemic lupus erythematosus. C4 reduction is commonly seen in immune complex induced nephritis, systemic lupus erythematosus, viral infections, lupus syndrome, cirrhosis, hepatitis, etc. Elevated C4 levels are commonly seen in the acute phase of wind dampness heat, nodular periarteritis, dermatomyositis, myocardial infarction, Reiter syndrome, and various types of polyarthritis. At present, the mainstream method for measuring C4 is immune scattering turbidimetry. During the measurement process, natural C4 with a certain concentration and purity is required as a calibration substance. Therefore, studying the separation and purification methods of C4 can provide a basis for the detection and research of related diseases. At present, although C4 separation methods have been continuously improved, their separation methods are relatively cumbersome, including pre-treatment, dialysis, ion exchange fiber chromatography, high-resolution anion chromatography, etc., and none of them can obtain high-purity natural C4 proteins. So there is an urgent need to develop a simple and efficient C4 separation technology to assist in the standardization of complement C4 determination and biological research.. Therefore, the CN116953041A patent provides a highly sensitive and fast responsive pH sensor and its working method. The specific preparation method is as follows:
For normal human samples, the target protein precipitate was obtained using a precipitant, and the precipitate containing the target protein was resuspended using a buffer solution (any of Tris, HEPES, MOPS, CHES, or MOPSO with a certain concentration of salt ions); The expressed complement C4 nanoantibody binds to a nickel affinity column, and the resuspended precipitate is loaded onto a nickel affinity column bound to complement C4 nanoantibody. After equilibrium and elution, natural complement C4 with high purity can be obtained.
This patented method utilizes protein fractionation precipitation, low-cost nano antibodies, and affinity reactions between antigens and nano antibodies. After two steps, the target protein can be obtained, reducing costs. Solved the problem of tedious pre-treatment for purifying C4 in existing technologies.

References
CN117304298A A method for separating natural protein of complement C4 using nano antibodies.