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Advantages, disadvantages, configuration methods and applications of Tris buffer

2019-03-29 来源:亚科官网
Buffer solution is a kind of solution that can resist the influence of a small amount of strong acid and alkali in the outside, and maintain the pH value of the system is basically unchanged. It has extremely important significance in the research work of biochemistry. Let's take a look at Tris buffer as an example to talk about Tris buffer.
Advantages of Tris Buffer
1 Because of the strong basicity of the Tris base, it is possible to use only one buffer system to prepare a buffer of a wide range of pH values ranging from acidic to alkaline;
2 Little interference with biochemical processes, no precipitation with calcium, magnesium ions and heavy metal ions.
Disadvantages of Tris Buffer
1 The pH value of the buffer is greatly affected by the solution concentration, the buffer is diluted ten times, and the changes of pH value is greater than 0.1;
2 The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer. ΔpKa/°C=-0.031. For example, the pH of the buffer at 8.4 °C is 8.4, and the pH at 37 °C is 7.4, so it must be Formulated at the temperature, the Tris-HCl buffer prepared at room temperature cannot be used at 0 °C ~ 4 °C;
3 Easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;
4 This buffer interferes with certain pH electrodes, so use an electrode compatible with Tris solution.
Configuration method of Tris buffer
There are two ways to prepare Tris-HCl buffer: separately prepare 0.05 mol/L Tris and 0.05 mol/L HCl solution, and then mix according to the volume listed in the common table. However, since the standard concentration of dilute hydrochloric acid is not easy to prepare, another method is commonly used: for example, a 1 L 0.1 mol/L Tris-HCl buffer solution is used: first, 12.11 g of Tris base is dissolved in 950 mL to 970 mL of deionized water. 4 N HCl was added dropwise with stirring, and the pH of the solution was measured with a pH meter to the desired pH, and then water was added to make up 1 L.
Tris buffer pH range
Tris Buffer is a widely used buffer in biochemical research. Its commonly used pH range is in the "neutral" range, such as:
Tris-HCl buffer: pH=7.5~8.5
Tris-phosphate buffer: pH=5.0~9.0
Application of Tris buffer solution
The pH of the buffer system is stabilized in the running buffer with glycine; the pH value is stabilized in the gel using Tris-HCl buffer system; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of Tris buffer can also be used for the formation of nematodes intermediate fibers; adding EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; replacing the pH-adjusted acid solution with acetic acid to obtain "TAE buffer", and replacing it with Boric acid can be obtained as a TBE buffer. These two buffers are commonly used in nucleic acid electrophoresis experiments.
Related Links: Tris Buffer
Edited by Suzhou Yacoo Science Co., Ltd.