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Luminol and its principle of illumination

2019-07-04 来源:亚科官网
 
The luminol sodium salt is a white to pale yellow crystalline powder obtained by reacting luminol with sodium hydroxide. Luminol is one of the most commonly used liquid chemiluminescent reagents. Since Albrecht first reported the chemiluminescence reaction of luminol with an oxidant in an alkaline solution in 1928, the study of the chemiluminescence system has been very active, making the chemiluminescence system used in many fields. Luminol sodium is one of the extensions of the application of luminol luminescence properties.
What is Luminol?
Luminol is one of the oldest and most commonly used reagents, which can be oxidized by peroxides under alkaline conditions, and emitting light. The redox reaction between luminol and peroxide requires a catalyst. For multivalent metal ions, peroxidases such as iron, horseradish peroxidase, etc., this method is commonly used to detect the content of peroxides, heavy metals, peroxidase, and the free radicals, toxic analysis and analytical methods based on peroxidase and glucose oxidase derived therefrom..
Under normal circumstances, luminol and hydrogen peroxide react very rapidly in the presence of certain catalysts. The most commonly used catalyst is a metal ion. In a large concentration range, the metal ion concentration is proportional to the luminescence intensity, so that the chemiluminescence analysis of some metal ions can be performed, and the organic compound containing the metal ion can be analyzed by using this reaction. The second is the use of organic compounds to inhibit the luminol chemiluminescence reaction, the determination of organic compounds that quench the chemiluminescence reaction. The third is to indirectly measure inorganic or organic compounds by coupling reaction.
The illumination principle of Luminol
First, sodium hypochlorite oxidizes luminol to cause it to emit light;
The second is that hydrogen peroxide reacts with sodium hypochlorite to form oxygen to oxidize luminol to cause it to emit light:
The equation for the reaction of sodium hypochlorite and hydrogen peroxide:
NaClO + H2O2 == NaCl + O2 + H2O
Luminol reacts with hydroxide to form a double anion (Dianion) which is oxidized by oxygen decomposed by hydrogen peroxide and the product is an organic peroxide. The peroxide is very unstable and immediately decomposes nitrogen (Luminol is oxidized by an organic oxidant such as dimethyl sulfoxide, which does not generate nitrogen, but forms a nitrogen-containing organic substance) to form an excited state of 3-aminophthalic acid. In the transition from the excited state to the ground state, the released energy exists in the form of photons, and the wavelength is in the blue portion of the visible light.
Related links: Luminol
Luminol sodium salt
Edited by Suzhou Yacoo Science Co., Ltd.