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The application of Tricine

2020-03-05 来源:亚科官网

Tricine(N-tris(hydroxymethyl)methylglycine), CAS: 5704-04-1, is a zwitterionic buffer used in biochemistry and molecular biology.The pH range of tricine is 7.4-8.8. Tricine is mainly used with SDS for small molecule protein electrophoresis to replace glycine . In biochemical research, it is often used in biochemical diagnostic 、kits DNA / RNA extraction kits and PCR diagnostic kits .

In the biochemical diagnostic kits 、 DNA / RNA extraction kits and PCR diagnostic kits, Tricine can inhibit nuclease activity and maintain cell osmotic pressure. maintain the integrity and high quality for DNA for a long time, and can be used for long fragment amplification. In addition, Tricine also has broad-spectrum bacteriostatic effect .

According to the published patent, Tricine acts as a co-chelating agent, chelating with the main chelating agent hydrazinyl bisaminonicotinamide for the preparation of MRI molecular imaging probes.

Tricine can also be used as an amphoteric electrolyte for the preparation of polyacrylamide gels. Polyacrylamide gels (PAGE) are widely used in biotechnology laboratories for processing biological samples to isolate the biomolecules present in the samples for identification, and in some cases, for quantitative separation.

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