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What are the production methods of N-Cyclohexyl-3-aminopropanesulfonic acid (CAPS)?

2020-04-21 来源:亚科官网

N-Cyclohexyl-3-aminopropanesulfonic acid (caps) is a biological buffer, mainly used in biochemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits. The preparation process of caps has been receiving attention. This article will introduce three production methods of caps.

The first methodDissolve cyclohexylamine in ethanol, then slowly add propionolactone, control the reaction temperature at about 50 ℃ through the feeding speed; after adding, continue the heat preservation reaction for 4 hours, filter and dry the caps crude. Then dissolve the crude 3- (cyclohexylamine) propanesulfonic acid (caps) in 100g of hot water and add ethanol, cool and crystallize, filter and dry to obtain the finished product of 3- (cyclohexylamine) propanesulfonic acid (caps).

This method has a simple process but the product solution of the produced caps has poor absorbance and has a problem of suspended matter, which results in low yield and poor quality during production.


The second methodDissolve propionolactone in N, N-dimethylformamide according to the weight ratio; add cyclohexylamine according to the preset speed, and control the reaction temperature at 30 ℃ through the preset speed of feeding; continue the heat preservation reaction for n hours after feeding, and then filter and dry to obtain crude caps product; dissolve the caps in hot water, then add ethanol, and then cool to 0 ℃, and then dry to obtain finished product of caps.

There are similarities in the preparation process of these two methods, the latter is an improvement of the former, and the yield of the resulting product is high and the quality is good.

The third methodDissolve 1,3-propane sultone with organic solvent A to obtain organic solution containing 1,3-propane sultone, dissolve cyclohexylamine with organic solvent B to obtain organic solution containing cyclohexylamine; The organic solution of propane sultone and the organic solution containing cyclohexylamine are simultaneously pumped into the micro-channel reactor to react to obtain the reaction liquid; then the obtained reaction liquid is precipitated by freezing crystallization of the crude caps and then passed through nitrogen pressure filtration to obtain the finished caps product .

The operation process of this method is simple and stable and controllable, and the production capacity and product yield are improved.

In summary, the preparation of caps mainly uses propane sultone and cyclohexylamine as raw materials. Through continuous development, standards such as simple process, high yield and good quality are the pursuit of caps production, which is conducive to high quality and high purity caps Production will help to further expand the application of caps.

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