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What Are The Applications of N-Cyclohexyl-3-aminopropanesulfonic acid

2020-04-29 来源:亚科官网

CAPS, the full name of N-Cyclohexyl-3-aminopropanesulfonic acid, is a biological buffer.  N-Cyclohexyl-3-aminopropanesulfonic acid is relatively stable in nature, with a pKa value of 10.4 at 25 ° C and a pH buffer range of 9.7-11.1. It is widely used in biochemical analysis and in vitro diagnostic industries.

N-Cyclohexyl-3-aminopropanesulfonic acid(CAPS)is mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, as a buffer for enzyme chemistry and HPLC separation of basic drugs. CAPS can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at a pH of 10.5, and dissolve in deionized water and adjust the pH to 11.0 for the purification of fibronectin.

In the field of capillary electrophoresis analysis, CAPS is used to prepare the running buffer (background electrolyte solution) in capillary electrophoresis as a terminal electrolyte, which can realize the online enrichment of 2-nitrobenzoic acid and 3-nitrobenzoic acid by electric pressure boost Separate.

In nucleic acid extraction, CAPS and CHAPS, CAPSO,etc. are used to prepare a solution for nucleic acid hybridization, which can reduce the non-specific hybridization products. The yield increases the specificity of nucleic acid hybridization while maintaining the yield of the target hybridization product. It has important value in the identification of specific pathogens, diagnosis of genetic diseases, and analysis of gene sequences.

N-Cyclohexyl-3-aminopropanesulfonic acid (CAPS) is also an important hydrophilic modifier. It can react with polyisocyanate under very mild reaction conditions and in the presence of a suitable neutralizing amine to prepare polyisocyanate products,which can emulsified into finely dispersed form in water and storage stable.

related link: CAPS

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