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What Role Does Guanidine Isothiocyanate Play In The Nucleic Acid Detection Kit For 2019-nCoV ?

2020-05-15 来源:亚科官网

Recently, a paper entitled "Expert Consensus for Clinical Application of New Coronavirus Nucleic Acid and Antibody Detection" published in the International Journal of Laboratory Medicine mentioned that in the collection process of 2019-nCoV,  it is recommended to use a sampling tube  with   virus inactivator such as guanidine isothiocyanate etc. to inactivate viruses and increase the detection rate.  So what is the role of guanidine isothiocyanate in the  nucleic acid Detection kits For 2019-nCoV , and how to inactivate the virus and improve the detection rate?

2019-nCoV is an RNA virus.The nucleic acids of various RNA viruses are biomolecules that are difficult to store stably due to self-degradation and biological enzyme-mediated degradation. Guanidine isothiocyanate is a kind of powerful protein denaturant, and has the characteristics of no RNase and DNase activity. The addition of guanidine isothiocyanate to the sampling tube can destroy the molecular structure of RNase, inactivate the nucleases that may be present in the sample, and play a role in stably storing the virus sample.

The nucleic acid detection process of 2019-nCoV includes sampling → preservation and delivery → virus inactivation → lysed nucleic acid extraction → detection, etc. Guanidine isothiocyanate also plays an important role in nucleic acid extraction. As the main component of the nucleic acid lysate, guanidine isothiocyanate can quickly break the virus, make the nucleoprotein and nucleic acid separate quickly, and at the same time inhibit the activity of the released nuclease, ensure the integrity of the primary structure of the nucleic acid, and improve the efficiency of nucleic acid extraction.

It can be seen that guanidine isothiocyanate has an important value in the detection of new coronavirus nucleic acid. 

With the development of large-scale nucleic acid detection, the demand for nucleic acid detection reagents and raw materials is also increasingly strong. It is understood that raw materials for nucleic acid detection reagents such as guanidine isothiocyanate and guanidine hydrochloride have become tight products.

Yacoo Science can provide guanidine isothiocyanate products with various packaging specifications and high quality  steadily , to help the development of nucleic acid detection reagent products, and promote the development of large-scale nucleic acid detection. Customers from all industries are welcome to consult.

Related links: Guanidine isothiocyanate