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What Is The Relationship Between HEPES-Na and HEPES? How Is HEPES-Na Prepared?

2020-06-03 来源:亚科官网

HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, its CAS number: 7365-45-9, HEPES is a zwitterionic buffer with a pka7.45 at 25 ℃ and a pH buffering range of 6.8-8.2. 

HEPES-Na,4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, sodium salt, its CAS number is: 75277-39-3. HEPES-Na and HEPES are very important biological buffers and are widely used in biopharmaceuticals, medical diagnostics and other fields.

The relationship between HEPES-Na and HEPES: 

HEPES-Na is also known as organic HEPES base, it and HEPES is a conjugate acid-base relationship. They are essentially the same, but the pH of them substances after dissolution is different.

The method of Preparing HEPES-Na:

According to published literature, HEPES-Na can be synthesized by 4-hydroxyethylpiperazine and sodium vinylsulfonate, or by high-pressure synthesis of isethionic acid, sodium hydroxide and 4-hydroxyethylpiperazine. The most extensive preparation method required by biological buffers is the reaction of HEPES with NaOH to produce HEPES-Na. The specific preparation steps are as follows:

Firstly, under the protection of nitrogen, HEPES and NaOH solids or solutions with a purity of 90-99% are reacted for 0.2-1 hour, the reaction temperature is 20-100°C. After the reaction is completed, the resulting product is decolorized, filtered, concentrated, Operations such as water removal, crystallization, washing, and drying are used to obtain HEPES-Na.

The preparation method is simple in process and convenient in operation, and the obtained HEPES-Na product has high yield and high product purity, and can meet the purity requirements of the biological buffer.

Yacoo Science. is mainly engaged in the development of biological buffer products. Over the years, it has been committed to the research and development and industrialization of biological buffers, and actively promotes the promotion and use of biological buffers in more fields. At present, the bio-buffer products of Yacoo Science.  have been widely used in in vitro diagnostics, bio-pharmaceuticals, bio-beauty, paints and other industries, and its downstream customers include many well-known Fortune 500 companies.

Related Links:HEPES-NaHEPES