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The in vitro diagnostic reagent materials used in liver function tests

2020-07-08 来源:亚科官网

 Liver function test is an important auxiliary method for the diagnosis of hepatobiliary system diseases. The basic items of liver function test usually include: alanine aminotransferase, aspartate aminotransferase, total bilirubin, serum adenosine deaminase, γ-glutamyl transpeptidase, alkali Phosphatase and other inspections. This article will introduce the raw materials and principles of in vitro diagnostic reagents commonly used in several liver function testing projects.

Alanine aminotransferase (ALT): Elevated alanine aminotransferase has been generally regarded as one of the most sensitive indicators for clinical diagnosis of hepatitis activity. The detection of serum alanine aminotransferase activity is currently the most commonly used measurement item for liver disease diagnosis and health examination.

 

The pyruvate oxidase method is the main method to detect the activity of alanine aminotransferase. Its principle is: ALT catalyzes the reaction of L-alanine and α-ketoglutarate to produce pyruvate, and pyruvate oxidizes under the catalysis of pyruvate oxidase,and hydrogen peroxide is generated. Hydrogen peroxide reacts with color reagent and 4-aminoantipyrine under the catalysis of peroxidase to form a red compound. The concentration of ALT enzyme activity is calculated based on the change in absorbance of the generated red compound. The developer is 3,3',5,5'-tetramethylbenzidine or phenol.

Aspartate aminotransferase (AST): Aspartate aminotransferase (AST), also known as aspartate aminotransferase, quantitatively detects the concentration of aspartate aminotransferase in the human body, which helps to understand the degree of human liver damage and determine the treatment effect. It is a routine liver function test project.

Chemiluminescence assay is a commonly used aspartate aminotransferase assay in recent years. Its principle is: aspartate aminotransferase catalyzes L-aspartic acid and α-ketoglutarate to produce L-glutamic acid; Glutamate is catalyzed by L-glutamate oxidase to produce hydrogen peroxide; under the action of peroxidase, hydrogen peroxide oxidizes chemiluminescence and emits light; the magnitude of the light signal is similar to that of aspartate aminotransferase The concentration is positively correlated, and the chemiluminescent substance is luminol.

 

The related kits for routine liver function tests often contain buffers, stabilizers, surfactants, color reagents and other components. The buffers include phosphate buffer, TRIS buffer, MES buffer, etc.; stabilizers mainly refer to bovine serum Albumin, casein; polysaccharides (sucrose, trehalose, etc.) and polyethylene glycol stabilizers; surfactants include Triton X-100, sodium lauryl sulfate, etc.; color developers include TMB, PNPP, DTNB, etc. These in vitro diagnostic reagent materials play a vital role in disease prevention and diagnosis.