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Application of proteinase K in nucleic acid extraction

2020-08-10 来源:亚科官网

Molecular diagnostics based on nucleic acid detection have shined in the prevention and control of epidemics. This is inseparable from the support of many high-quality raw materials, and proteinase K is an indispensable member.

Proteinase K is a serine protease with wide cleavage activity, and its , CAS No.is 39450-01-6 . Proteinase K has a wide range of substrate specificity and excellent stability, and is active in a wide pH range (pH4pH12.5) and high temperature (5070℃). In addition, proteinase K can stably exist in a variety of chemical reagents that can denature proteins, such as SDS, urea, EDTA chelating agents and sulfhydryl reagents (such as trypsin inhibitor or chymotrypsin inhibitor), and has the ability to solubilize proteins.

 

Because of these characteristics, proteinase K is used in medical treatment (killing viruses and microorganisms), food (meat tenderization), leather (hair softening), winemaking (clarification of wine), amino acid preparation (degradation of feathers), nucleic acid extraction, and in situ hybridization. It has a wide range of applications, and nucleic acid extraction is its most common application. The application of proteinase K in nucleic acid extraction involves many fields such as the prevention of food-borne diseases, the prevention of agricultural diseases and insect pests, forensic gene identification and comparison, and molecular biology experiment research. Application objects include prokaryotes, human blood samples and tissues, animal and plant tissues, parasites, microorganisms and other categories.

Proteinase K can be used for the isolation of insect genomic DNA. The method is simple to operate and has a high success rate. The extracted DNA is of high quality, which provides a basis for further research on plant insect resistance. Proteinase K is used for the extraction of plant cell DNA, which can digest most of the proteins in cells and ensure the completeness and purity of the nucleic acid structure.

 

In SARS-CoV-2 nucleic acid detection reagents, proteinase K is an important component of the virus sampling solution. It can crack and inactivate the coat protein of the virus, making the sample non-infectious during the transportation and detection stage, ensuring the safety of the operator, and protease K can degrade RNase, prevent the degradation of viral RNA, is beneficial to nucleic acid detection, and is known as a nucleic acid extraction tool.

Proteinase K plays an important role in the diagnosis and prevention and control of the epidemic. It is an indispensable anti-epidemic material. As a national high-tech enterprise dedicated to the research and development, production and supply of in vitro diagnostic reagents, Yacoo Science can provide stable quality and reliable protease K, the article number is D0002, welcome to inquire.

Related link: Proteinase K